Calculated abundance for paired-end sequencing

[JingliangShi]

In ARGs-OAP V3.2, each sample produces paired-end files corresponding to two results of ARGs abundance. I wonder if the abundance of ARGs from each sample is the sum of the abundances of the paired-end files. Thank you for your reply.

[xinehc]

if your paired files ends with _1|_2, _R1|_R2 or _fwd|_rev, a sample should show up only once in your output file.

if the abundance is defined as copies per 16s/cell/Gb, then you cannot simply sum the abundance of _1 and _2 up.

[JingliangShi]

if your paired files ends with _1|_2, _R1|_R2 or _fwd|_rev, a sample should show up only once in your output file.

if the abundance is defined as copies per 16s/cell/Gb, then you cannot simply sum the abundance of _1 and _2 up.

Thanks for your answer! Can we calculate the average value of them as the relative abundance (copies per 16s/cell/Gb)?

[xinehc]

yes, _1 and _2 should have similar abundance