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scripts and protocols for Docker/Garroway Labs to work from fastq to VCF

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training_g4

This repository contains scripts and protocols for Docker/Garroway Labs at the University of Manitoba as they trainees go from raw fastq files through to VCF

The steps we will be following:

  • Run a script to cp files from projects to scratch
  • Run fastqc on raw reads
  • Run Multiqc to view all records together
  • Run trimmomatic to remove adapters
  • Run fastqc and multiqc again
  • Run bowtie2 to map reads to the genome (generate bam files)
  • Run picard to remove duplicate reads
  • Run platypus to call SNPs (generate vcf file)

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