This repository contains scripts and protocols for Docker/Garroway Labs at the University of Manitoba as they trainees go from raw fastq files through to VCF
- Run a script to
cp
files fromprojects
toscratch
- Run
fastqc
on raw reads - Run
Multiqc
to view all records together - Run
trimmomatic
to remove adapters - Run
fastqc
andmultiqc
again - Run
bowtie2
to map reads to the genome (generate bam files) - Run
picard
to remove duplicate reads - Run
platypus
to call SNPs (generate vcf file)