On-Target and Off-Target Scoring Algorithms for CRISPR gRNAs

CRISPR Guide RNA Designer

Creates a construct developed by the Niles Lab at MIT, designed to deliver a 3' UTR post-transcriptional regulatory element payload to a specific given gene in Plasmodium falciparum.

Fork of Azimuth (Machine Learning-Based Predictive Modelling of CRISPR/Cas9 guide efficiency), updated to work with Python 3.10+

⛓️ Automating construction of oligo library sequences for oligo array synthesis.