This research study dove into understanding the gut microbiome of covid positive patients at different severity levels using long-read 16S sequencing with the following parameters:
- ONT R9.4.1 chemistry
- Dorado v0.5.0 super accuracy basecalling
- Emu v3.4.5 taxonomic classification and abundance estimation
Along with the long-read 16S sequencing data, there is a plethora of metadata that goes alongside each deidentified patient. We intend and encourage folks to go through this data further and use this vignette as a tool for processing further 16S long-read datasets using R.
All the code used in the analysis and visualization of Emu microbiome outputs can be found in our documentation.